HPLC Method Development and Validation for the Estimation of Amlodipine and Atorvastatin in Bulk and Formulation

In the present research work, a successful attempt was made for Validated UV and HPLC method development estimation of Amlodipine Atorvastatin in marketed formulation which developed by experimentation based on thorough literature survey ascertained statistical parameters sampling. The simplicity, rapidity, accurate reproducibility proposed methods completely fulfill objective work drug formulation. Liquid chromatographic system from waters comprising manual injector, Waters 515 binary pump constant flow pressure delivery U.V. detector connected to data ace software controlling instrumentation as well processing generated were used. isocratic mobile phase consisted 20 mM KH2PO4: Acetonitrile (pH 3 with OPA) ratio 20:80 v/v at rate 1.0 ml min-1. A thermo C-18 column (4.6 x 250mm, 5μ particle size) used stationary phase, 237.0 nm selected detection wavelength UV-vis. detector. found be linear range 1-5 μg/ml & 5-25 correlation coefficient close one amlodipine atorvastatin respectively. Precision determined repeatability, Intermediate precision drugs. robustness checked changing deliberate variation solvent. Simplicity, Rapidly Reproducibility this work. 
 Keywords: Amlodipine, Atorvastatin, Method development, HPLC, Validation